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ACS BIOCHEMISTRY Solved Exam |100% Pass| Henderson-Hasselbach Equation *Ans* pH = pKa + log ([A-] / [HA]) FMOC Chemical Synthesis *Ans* Used in synthesis of a growing amino acid chain to a polystyrene bead. FMOC is used as a protecting group on the N-terminus. Salting Out (Purification) *Ans* Changes soluble protein to solid precipitate. Protein precipitates when the charges on the protein match the charges in the solution. Size-Exclusion Chromatography *Ans* Separates sample based on ...

how much BSA is needed to prepare 50 mL of 5 mg/ml of BSA (w/v)? correct answers (50 mL)(5 mg,mL) = 250 mg a technician is assigned the task of inserting a gene, LanA, into a plasmid to ultimately be expressed as a therapeutic protein in E coli. label the following steps (1-4) in the correct order the technician must perform them to accomplish her task. correct answers 1) design primers to amplify the lanA sequence using polymerase chain reaction (PCR) and perform the PCR reaction 2) diges...

how much BSA is needed to prepare 50 mL of 5 mg/ml of BSA (w/v)? correct answers (50 mL)(5 mg,mL) = 250 mg a technician is assigned the task of inserting a gene, LanA, into a plasmid to ultimately be expressed as a therapeutic protein in E coli. label the following steps (1-4) in the correct order the technician must perform them to accomplish her task. correct answers 1) design primers to amplify the lanA sequence using polymerase chain reaction (PCR) and perform the PCR reaction 2) diges...

What is an example of a product easily found in nature? - Answer- Antibiotic In a biotechnology lab, what is tap water is best used for? - Answer- Washing glassware. What is the order of amino acids on a polypeptide chain encoded by? - Answer- mRNA How do DNA molecules vary from one species to another? - Answer- Number of genes and non-coding regions. What is agar? - Answer- Solid media on which bacteria grow. What part of an amino acid differs from one to the other? - Answer- R g...

Biochemistry Exam #2 (Module 3) questions and answers 100% verified. (Exam question 21) Dialysis - correct ates small molecules from large molecules (Exam question 30) When a protein is 'salted-in', it precipitates and is removed form a solution. - correct answers.False When we purify protein, we are going against the natural tendency toward disorder (we are fighting against entropy). - correct answers.True When centrifuging sub-cellular structures, what organ...

FL 5 AAMC MCAT EXAM 2024 What is the chemical structure of a component found in four of the five cofactors used by Na+- NQR? -Answer-says it is composed of flavin structure is a nucleotide base structure B could be structure C also could be structure D is an amino acid down between B and C to help, look at the captioned note on the table. it says Fl represents flavin which is the redox active center in four of the five cofactors of Na+-NQR C has an uniquinone AND HERE IS IS MENTIONED T...

What is an example of a product easily found in nature? Antibiotic In a biotechnology lab, what is tap water is best used for? Washing glassware. What is the order of amino acids on a polypeptide chain encoded by? mRNA How do DNA molecules vary from one species to another? Number of genes and non-coding regions. What is agar? Solid media on which bacteria grow. What part of an amino acid differs from one to the other? R group How can colorless prot...

Biotechnician Assistant Credentialing Practice Exam Latest Update Graded A After performing the PCR reaction with the primers she designed to amplify lanA, the same technician found that her PCR reaction contained two distinct bands, one at the expected size of 1200 kilobase pairs, and another unexpected band at 2000 kilobase pairs. What is the most likely reason there is an unexpected band in her PCR reaction? She used an annealing temperature that was too low In a biotechnology lab, tap wate...

Western blotting a. Proteins from SDS-PAGE are transferred to a nitrocellulose membrane using electrical current a. membrane is blocked so antibody will not bind nonspecifically b. Primary antibody is added and incubated with the membrane c. To detect antibodies that have bound to protein a secondary anti-immunoglobulin antibody coupled to a reporter a. Excess secondary antibody is washed off the membrane b. Substrate is added that precipitates upon reaction c. Reveals visible band where...

AAMC FL 1 Exam Questions and Answers 2024 If Reaction 2 (Figure 4) is repeated with HCl and the compound shown below, which of the following compounds is NOT a direct product (without rearrangement)? -Answer-Compound A requires a hydride shift to form- therefore not a direct product. NOT an SN2 reaction because of tertiary alcohol- it is SN1 What is the energy of the photons emitted by the LED at a frequency of 610 THz? (Note: h = 6.6 × 10-34 J·s) -Answer-The photon energy is the energy...