QPCR Study guides, Class notes & Summaries
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MB (ASCP) Review & Exam (latest update 2023) Questions Part II Already Graded A+
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What is the Beer-Lambert Law? - Answer the linear relationship between absorbance and concentration of an absorbing species. 
 
What is the Beer-Lambert Law equation? - Answer A = ebc 
 
A: absorbance 
e: molar absorptivity 
c: concentration 
b: path length 
 
DNA and RNA are absorbed at: - Answer 260nm 
 
Proteins are absorbed at: - Answer 280nm 
 
What is the melting temperature equation? - Answer Tm = (4 x # of GC pairs) + (2 x # of AT pairs) 
 
Dilutions equation - Answer V1C1 = V2C2 
 
V1:...
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MB (ASCP) Review & Exam Questions Part III and Answers with Complete Solutions, Graded A+ 2023|2024 UPDATE
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MB (ASCP) Review & Exam Questions Part III and Answers with 
Complete Solutions, Graded A+ 
1 / 14 
1. PCR can test for what diseases?: Hep - B 
Herpes simplex 1 
Herpes simplex 2 
HIV 
HPV 
Epstein-barr 
Cytomegalovirus 
Cystic fibrosis 
Huntingtons 
Bk virus 
Fragile X 
Parovirus 
2. qPCR can test for what diseases?: Herpes simplex 1 
Herpes simplex 2 
Epstein-barr 
HPV 
HIV 
Cytomegalovirus 
Bk virus 
VZV 
3. RT PCR can test for what diseases?: HIV 
Influenza 
Coronavirus 
Ewing sarcoma 
Syno...
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ASCP MB Exam Questions|2023 LATEST UPDATE|GUARANTEED SUCCESS
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Which PCR method has the highest specificity? 
 
A. qPCR 
B. Touchdown PCR 
C. Nested PCR 
D. Two-step PCR 
Nested PCR 
 
 
 
Given the anti-sense strand of DNA: 5'-AATTGCCGACATAGAT-3' which is the appropriate sense strand? 
 
A. 5'-ATCTATGTCGGCAATT-3' 
B. 5'-TTAACGGCTGTATCTA-3' 
C. 5'-AATTGCCGACATAGAT-3' 
D. 5'-GAGCACGCTATCTTAT-3' 
5'-ATCTATGTCGGCAATT-3' 
 
 
 
During DNA replication, the 3' -OH of the growing DNA chain attacks which phosphate of an incoming nucleotide? 
A. Alpha 
...
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MB ASCP VERIFIED SOLUTIONS 100% GUARANTEE PASS
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MB ASCP VERIFIED 
SOLUTIONS 100% 
GUARANTEE PASS 
What genes would be screened in a lung cancer panel? - ANSWER KRAS, EGFR, ALK 
In a retrovirus, the RNA acts as: - ANSWER Genome and mRNA 
To what does the AcrAB gene product create resistance to? - ANSWER Tetracycline 
What gene is measured following the treatment with imatinib(Gleevec)? - ANSWER 
BCR/Abl 
Blood drawn into what type of tube is compatible for genetic testing? - ANSWER K2- 
EDTA tube 
Primer dimers are due to what complimentary...
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AAB Molecular Diagnostics Testing with complete solutions 2023
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AAB Molecular Diagnostics Testing with complete solutions 2023This DNA amplification method amplifies the nucleic acid used as a probe? 
Ligase Chain Reaction (LCR) 
 
 
 
What enzymes are used in Ligase Chain Reactions? 
DNA polymerase and DNA ligase 
 
 
 
What is the function of DNA polymerase in Ligase Chain Reaction? 
Is used to initiate DNA polymerase. 
 
 
 
What is the key advantage of using Ligase Chain Reaction instead of Polymerase Chain Reaction? 
Better specificity 
 
 
 
How does L...
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MB (ASCP) 2022 - Translocations, Diseases, Pharma, Polymerases, & more
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MB (ASCP) 2022 - Translocations, Diseases, Pharma, Polymerases, & more Chronic Myeloid Leukemia (CML) t(9;22) BCR-ABL translocation 
Ewing sarcoma t(11;22) EWS-FLI1 
Acute Lymphocytic Leukemia (ALL) t(9;22) BCR-ABL p190 protein 
Follicular Lymphoma t(14;18) BCL2 translocation 
Mantle Cell Lymphoma t(11;14) bcl-1 translocation CCND1-IgH 
Burkitt Lymphoma t(8;14) MYC gene translocation 
Rhabdomyosarcoma (alveolar); Cancer/solid tumor t(2;13) or t(1;13) PAX3-FKHR and PAX7-FKHR "ARMS" translocatio...
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2023/2024 MB (ASCP) Review & Exam Questions Part III and Answers With Complete Solutions Graded A+
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1 / 14 
1. PCR can test for what diseases?: Hep - B 
Herpes simplex 1 
Herpes simplex 2 
HIV 
HPV 
Epstein-barr 
Cytomegalovirus 
Cystic fibrosis 
Huntingtons 
Bk virus 
Fragile X 
Parovirus 
2. qPCR can test for what diseases?: Herpes simplex 1 
Herpes simplex 2 
Epstein-barr 
HPV 
HIV 
Cytomegalovirus 
Bk virus 
VZV 
3. RT PCR can test for what diseases?: HIV 
Influenza 
Coronavirus 
Ewing sarcoma 
Synovial sarcoma 
Hep - C 
4. Multiplex PCR can test for what diseases?: B. pertussis 
M. pneumo...
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MB (ASCP) Review & Exam Questions Part II Questions and answers 2023
- Exam (elaborations) • 16 pages • 2023
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MB (ASCP) Review & Exam Questions Part II Questions and answers 2023 
What is the Beer-Lambert Law? 
 
the linear relationship between absorbance and concentration of an absorbing species. 
 
 
 
What is the Beer-Lambert Law equation? 
 
A = ebc 
 
A: absorbance 
e: molar absorptivity 
c: concentration 
b: path length 
 
 
 
DNA and RNA are absorbed at: 
 
260nm 
 
 
 
Proteins are absorbed at: 
 
280nm 
 
 
 
What is the melting temperature equation? 
 
Tm = (4 x # of GC pairs) + (2 x # of AT p...
-
(MB) ASCP Practice Exam Questions Answered 100% correct
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(MB) ASCP Practice Exam Questions Answered 100% correct 
Which of the following is not a component of a nucleotide? 
Phosphate group 
Anti-codon 
Ribose sugar 
Nitrogen base 
Anti-codon 
 
 
 
According to Chargaff's rule of base pairing, adenine pairs with: 
Thymine 
 
 
 
What genes would be screened in a breast cancer panel? 
HER2, ERBB2, BRCA1 
 
 
 
Next Generation Sequencing uses: 
Short sequence reads 
 
 
 
Purines and pyrimidines differ from each other in that: 
Purines have two rings...
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(MB) ASCP Practice Exam Questions and Answers Solved Correctly
- Exam (elaborations) • 44 pages • 2023
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Which of the following is not a component of a nucleotide? 
Phosphate group 
Anti-codon 
Ribose sugar 
Nitrogen base Anti-codon 
According to Chargaff's rule of base pairing, adenine pairs with: Thymine 
What genes would be screened in a breast cancer panel? HER2, ERBB2, BRCA1 
Next Generation Sequencing uses: Short sequence reads 
Purines and pyrimidines differ from each other in that: Purines have two rings; pyrimidines 
have one ring 
The purines are: 
Cytosine and uracil 
Adenine and thymin...
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