Recombinant Study guides, Class notes & Summaries

PURIFICATION OF HEXAHISTIDINE TAGGED GFP PROTEIN FROM TRANSFORMED E.COLI XL1 CELLS USING IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY Lee Jia Yi (12) Abstract In this study, a recombinant histidine-tagged green fluorescence protein(His6-GFP) was produced by E.coli XL1 cells followed by immobilized metal affinity chromatography (IMAC) using Nickel-agarose beads for purification. The GFP template was first amplified from pE-GFP vector using polymerase chain reaction (PCR). The primers c...

Test bank for Modern Blood Banking and Transfusion Practices 7th Edition by Denise M. Harmening Table of Contents Chapter 1. Red Blood Cells and Platelet Preservation: Historical Perspectives and Current Trends ............................................. 3 Answer Section .......................................................................................................................................................................... 8 Chapter 2. Basic Genetics ............................

NRNP 6531 / NRNP6531 Advanced Primary Care of Adults Final Exam Quiz Bank Rated A |Questions and Answers with Rationales | Latest 2022/2023 1. A patient was initially treated as an outpatient for pneumonia and then after 2 weeks was hospitalized after no improvement was evident. The patient continues to show no improvement after several antibiotic regimens have been attempted. What is the next step in managing this patient? ~ Answer: D Rat: Patients who do not respond to antibiotic th...

What does recombinant DNA technology involve? - Answer Transfer of fragments of DNA from one organisms or species to another What can recombinant DNA technology be used for? - Answer Medicine: producing large amounts of a gene product e.g. protein Agriculture: genetically engineer crops Why can DNA be transferred between organisms? - Answer Genetic code is universal and so are the transcription and translation factors Give three examples of how fragments of DNA can be produced...

Why is it important to use the same restriction endonuclease on DNA samples in an experiment? CORRECT ANSWER Access same base sequence So get fragments with required gene **Suggest why scientists insert a plasmid with EGFP (a gene that codes for bright protein) CORRECT ANSWER Those cells that do not glow brightly have not taken up the plasmid Name the enzyme used to cut a gene from human DNA CORRECT ANSWER Restriction endonuclease Suggest reasons why very few live births result from t...

Table of contents 1. A Brief History of Microbiology 2. The Chemistry of Microbiology 3. Cell Structure and Function 4. Microscopy, Staining, and Classification 5. Microbial Metabolism 6. Microbial Nutrition and Growth 7. Microbial Genetics 8. Recombinant DNA Technology 9. Controlling Microbial Growth in the Environment 10. Controlling Microbial Growth in the Body: Antimicrobial Drugs 11. Characterizing and Classifying Prokaryotes 12. Characterizing and Classifying Eukaryotes 13. Ch...

AQA A-Level Biology- Recombinant DNA Technology 3.8.4.1 What does recombinant DNA technology involve? organisms or species to another What can recombinant DNA technology be used for? gene product e.g. protein Agriculture: genetically engineer crops Why can DNA be transferred between organisms? transcription and translation factors Transfer of fragments of DNA from one Medicine: producing large amounts of a Genetic code is universal and so are the Give three examples o...

Principles of Biochemistry 4th Edition Testbank(Horton) Chapter 1 Introduction to Biochemistry Chapter 2 Water Chapter 3 Amino Acids and the Primary Structures of Proteins Chapter 4 Proteins: Three-Dimensional Structure and Function Chapter 5 Properties of Enzymes Chapter 6 Mechanisms of Enzymes Chapter 7 Coenzymes and Vitamins Chapter 8 Carbohydrates Chapter 9 Lipids and Membranes Chapter 10 Introduction to Metabolism Chapter 11 Glycolysis Chapter 12 Gluconeogenesis, The Pentose Pho...

WGU D027 Course Material Questions and Answers Latest Update, Graded A A 23-year-old woman comes in for prenatal counseling. While completing her family history, she reports her brother has cystic fibrosis. She does not know if she is a carrier. She asks if her children will be affected by the disease. What is an accurate way to determine the likelihood of this patient's children being affected? Correct Answer- A genetic or a carrier test - Cystic fibrosis is a hereditary disease that im...

What modification neutralizes the charges on histones and looses up the interactions between histones and DNA? - Answer- ACETYLATION Two types of post-transcriptional modifications that take place in the mRNA of eukaryotes - Answer- the addition of a CAP at the 5' end of the transcript and the addition of a POLY A sequence at the 3' end of the message Nucleic acid blotting is widely used in recombinant DNA technology. In a Southern blot one generally - Answer- Hybridizes filter-bound DNA...